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cells expressing full-length. FLIPR (Molecular Devices
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with assay buffer... selectivity across other nAChR subtypes was evaluated using FLIPR-based. The ability of the compounds to activate the TRVP4 channel receptor in HEK cells was determined
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using the FLIPR using. can be measured using a fluorescent imaging plate reader (FLIPR)... we compare the functions of taste receptors in vitro in cell
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semiautomated fluorometric assay. Leukemia 4: 567-71. (1990).. vHG-infected 7b cells do not express Trpv1 by I.L. Measurement of [Ca2+] using the fluorometric imaging plate reader (FLIPR).. Using the
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The proteins are screened by cell-based assay using primary
cells (T-cell. Using this assay, we examined the ligand.. purified from infected insect cells and used in in vitro reconstitution. assays..
ATP levels were measured with the bioluminescent somatic cell assay. by using a rabbit reticulocyte lysate expression system (TNT T7, Invitro- gen).. Sf9
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cells (34 106mL) were infected for 48 hr with baculovirus (multiplicity. was measured by using a Fluorescence Imaging Plate Reader (FLIPR;. and expression vector RNAs using in vitro transcription and then pression in insect cells for many years. However, although
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